Melanoma Antigen Family D, 4 (MAGED4) (AA 51-741) antibody

Catalog No. ABIN7601938

The Rabbit Polyclonal anti-Melanoma Antigen Family D, 4 antibody has been validated for WB, ELISA, ICC, IF, FACS and IHC. It is suitable to detect Melanoma Antigen Family D, 4 in samples from Human.

Quick Overview for Melanoma Antigen Family D, 4 (MAGED4) (AA 51-741) antibody (ABIN7601938)

Target: Melanoma Antigen Family D, 4 (MAGED4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 51-741

Purpose: Anti-MAGED4 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MAGED4 Antibody (ABIN7601938). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MAGED4 recombinant protein (Position: F51-R741).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Melanoma Antigen Family D, 4 (MAGED4)

Alternative Name: MAGED4

Background:

Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: MAGE family member D4 is a protein that in humans is encoded by the MAGED4 gene. MAGED4, originally termedMAGEE1, is a member ofMAGE (Melanoma associated antigen)super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556, 24966945). Three isoforms(MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of humanMAGED4 protein detects a band of 90-100 kDa, which islargerthan the calculated molecularweight. The slower electrophoretic mobility may be due to abnormal spatial conformation ofMAGED4 protein caused by abundance of proline in composition (PMID: 24966945).

Molecular Weight: 100 kDa

Gene ID: 728239