ITSN1 antibody (AA 522-1721)

Catalog No. ABIN7601964

Product Details anti-ITSN1 Antibody

Target: ITSN1 (Intersectin 1 (ITSN1))

Binding Specificity: AA 522-1721

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITSN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Anti-Intersectin 1/ITSN1 Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-Intersectin 1/ITSN1 Antibody Picoband® (ABIN7601964). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Intersectin 1/ITSN1 recombinant protein (Position: A522-P1721).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, H., Antonarakis, S. E. The SH3D1A gene maps to human chromosome 21q22.1-q22.2. Cytogenet. Cell Genet. 78: 213-215, 1997. 2. He, G., Wang, H.-R., Huang, S.-K., Huang, C.-L. Intersectin links WNK kinases to endocytosis of ROMK1. J. Clin. Invest. 117: 1078-1087, 2007. 3. Irie, F., Yamaguchi, Y. EphB receptors regulate dendritic spine development via intersectin, Cdc42 and N-WASP. Nature Neurosci. 5: 1117-1118, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ITSN1

Target: ITSN1 (Intersectin 1 (ITSN1))

Alternative Name: ITSN1 (ITSN1 Products)

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: Intersectin-1 is a protein that, in humans, is encoded by the ITSN1 gene. The protein encoded by this gene is a cytoplasmic membrane-associated protein that inly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far.

Molecular Weight: 150 kDa

Gene ID: 6453

UniProt: Q15811

Pathways: Neurotrophin Signaling Pathway