SUPT5H antibody (AA 533-1087)

Catalog No. ABIN7601985

This Rabbit Polyclonal antibody specifically detects SUPT5H in WB, ELISA, IHC, IF, ICC and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for SUPT5H antibody (AA 533-1087) (ABIN7601985)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT5H antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SUPT5H Antibody

Binding Specificity: AA 533-1087

Purpose: Anti-SPT5/SUPT5H Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SPT5/SUPT5H Antibody Picoband® (ABIN7601985). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPT5/SUPT5H recombinant protein (Position: Q533-A1087).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chiang, P.-W., Fogel, E., Jackson, C. L., Lieuallen, K., Lennon, G., Qu, X., Wang, S.-Q., Kurnit, D. M. Isolation, sequencing, and mapping of the human homologue of the yeast transcription factor, SPT5. Genomics 38: 421-424, 1996. 2. Chiang, P.-W., Stubbs, L., Zhang, L., Kurnit, D. M. Isolation of murine SPT5 homologue: completion of the isolation and characterization of human and murine homologues of yeast chromatin structural protein complex SPT4, SPT5, and SPT6. Genomics 47: 426-428, 1998. 3. Guo, S., Yamaguchi, Y., Schilbach, S., Wada, T., Lee, J., Goddard, A., French, D., Handa, H., Rosenthal, A. A regulator of transcriptional elongation controls vertebrate neuronal development. Nature 408: 366-369, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Alternative Name: SUPT5H

Background:

Synonyms: Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K (+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42, MIG1

Tissue Specificity: Ubiquitously expressed.

Background: Transcription elongation factor SPT5 is a protein that in humans is encoded by the SUPT5H gene. Enables enzyme binding activity and protein heterodimerization activity. Involved in positive regulation of macroautophagy, regulation of RNA metabolic process, and transcription elongation from RNA polymerase II promoter. Located in nucleoplasm. Part of DSIF complex.

Molecular Weight: 150 kDa

Gene ID: 6829

UniProt: O00267