UBE2S antibody (AA 6-155)

Catalog No. ABIN7602116

This anti-UBE2S antibody is a Rabbit Polyclonal antibody detecting UBE2S in WB, ELISA, IF, FACS and ICC. Suitable for Human and Rat.

Quick Overview for UBE2S antibody (AA 6-155) (ABIN7602116)

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2S antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-UBE2S Antibody

Binding Specificity: AA 6-155

Purpose: Anti-UBE2S Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-UBE2S Antibody Picoband® (ABIN7602116). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UBE2S recombinant protein (Position: E6-H155).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/10/2006. 2. Jung, C.-R., Hwang, K.-S., Yoo, J., Cho, W.-K., Kim, J.-M., Kim, W. H., Im, D.-S. E2-EPF UCP targets pVHL for degradation and associates with tumor growth and metastasis. Nature Med. 12: 809-816, 2006. 3. Liu, Z., Diaz, L. A., Haas, A. L., Giudice, G. J. cDNA cloning of a novel human ubiquitin carrier protein: an antigenic domain specifically recognized by endemic pemphigus foliaceus autoantibodies is encoded in a secondary reading frame of this human epidermal transcript. J. Biol. Chem. 267: 15829-15835, 1992.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for UBE2S

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Alternative Name: UBE2S

Background:

Synonyms: Collagen alpha-1 (XVII) chain, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2, 120 kDa linear IgA dermatosis antigen, Linear IgA disease antigen 1, LAD-1, 97 kDa linear IgA bullous dermatosis antigen, 97 kDa LAD antigen, 97-LAD, Linear IgA bullous disease antigen of 97 kDa, LABD97, COL17A1, BP180, BPAG2

Tissue Specificity: Detected in skin. In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells. Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.

Background: This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.

Molecular Weight: 24 kDa

Gene ID: 27338

UniProt: Q16763

Pathways: Ubiquitin Proteasome Pathway