CRABP1 antibody (AA 60-118)

Catalog No. ABIN7602147

This Rabbit Polyclonal antibody specifically detects CRABP1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for CRABP1 antibody (AA 60-118) (ABIN7602147)

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRABP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CRABP1 Antibody

Binding Specificity: AA 60-118

Purpose: Anti-CRABP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CRABP1 Antibody Picoband® (ABIN7602147). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CRABP1 recombinant protein (Position: R60-E118).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Flagiello, D., Apiou, F., Gibaud, A., Poupon, M. F., Dutirllaux, B., Malfoy, B. Assignment of the genes for cellular retinoic acid binding protein 1 (CRABP1) and 2 (CRABP2) to human chromosome band 15q24 and 1q21.3, respectively, by in situ hybridization. Cytogenet. Cell Genet. 76: 17-18, 1997. 2. Geurts van Kessel, A., de Leeuw, H., Dekker, E. J., Rijks, L., Spurr, N., Ledbetter, D., Kootwijk, E., Vaessen, M. J. Localization of the cellular retinoic acid binding protein (CRABP) gene relative to the acute promyelocytic leukemia-associated breakpoint on human chromosome 15. Hum. Genet. 87: 201-204, 1991. 3. Guidez, F., Parks, S., Wong, H., Jovanovic, J. V., Mays, A., Gilkes, A. F., Mills, K. I., Guillemin, M.-C., Hobbs, R. M., Pandolfi, P. P., de The, H., Solomon, E., Grimwade, D. RAR-alpha-PLZF overcomes PLZF-mediated repression of CRABPI, contributing to retinoid resistance in t(11,17) acute promyelocytic leukemia. Proc. Nat. Acad. Sci. 104: 18694-18699, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CRABP1

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Alternative Name: CRABP1

Background:

Synonyms: DAZ-associated protein 1, Deleted in azoospermia-associated protein 1, DAZAP1

Tissue Specificity: Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.

Background: Cellular retinoic acid-binding protein 1 is a protein that in humans is encoded by the CRABP1 gene. This gene encodes a specific binding protein for a vitamin A family member and is thought to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid at specific sites within the nucleus, which may contribute to vitamin A-ed differentiation in epithelial tissue.

Molecular Weight: 16 kDa

Gene ID: 1381

UniProt: P29762