ALG3 antibody (AA 61-438)

Catalog No. ABIN7602167

Product Details anti-ALG3 Antibody

Target: ALG3 (ALG3, alpha-1,3- mannosyltransferase (ALG3))

Binding Specificity: AA 61-438

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALG3 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Purpose: Anti-ALG3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ALG3 Antibody Picoband® (ABIN7602167). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALG3 recombinant protein (Position: I61-H438).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Denecke, J., Kranz, C., Kemming, D., Koch, H.-G., Marquardt, T. An activated 5-prime cryptic splice site in the human ALG3 gene generates a premature termination codon insensitive to nonsense-mediated mRNA decay in a new case of congenital disorder of glycosylation type Id (CDG-Id). Hum. Mutat. 23: 477-486, 2004. 2. Denecke, J., Kranz, C., von Kleist-Retzow, J. C., Bosse, K., Herkenrath, P., Debus, O., Harms, E., Marquardt, T. Congenital disorder of glycosylation type Id: clinical phenotype, molecular analysis, prenatal diagnosis, and glycosylation of fetal proteins. Pediat. Res. 58: 248-253, 2005. 3. Korner, C., Knauer, R., Stephani, U., Marquardt, T., Lehle, L., von Figura, K. Carbohydrate deficient glycoprotein syndrome type IV: deficiency of dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase. EMBO J. 18: 6816-6822, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ALG3

Target: ALG3 (ALG3, alpha-1,3- mannosyltransferase (ALG3))

Alternative Name: ALG3 (ALG3 Products)

Background:

Synonyms: Growth arrest and DNA damage-inducible protein GADD45 gamma, Cytokine-responsive protein CR6, DNA damage-inducible transcript 2 protein, DDIT-2, GADD45G, CR6, DDIT2

Background: Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase is an enzyme that, in humans, is encoded by the ALG3 gene. This gene encodes a member of the ALG3 family. The encoded protein catalyses the addition of the first dol-P-Man derived mannose in an alpha 1,3 linkage to Man5GlcNAc2-PP-Dol. Defects in this gene have been associated with congenital disorder of glycosylation type Id (CDG-Id) characterized by abnormal N-glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 50-55 kDa

Gene ID: 10195

UniProt: Q92685