AXIN2 antibody (AA 614-843)

Catalog No. ABIN7602177

This Rabbit Polyclonal antibody specifically detects AXIN2 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for AXIN2 antibody (AA 614-843) (ABIN7602177)

Target: AXIN2 (Axin 2 (AXIN2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AXIN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-AXIN2 Antibody

Binding Specificity: AA 614-843

Purpose: Anti-Axin 2/AXIN2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Axin 2/AXIN2 Antibody Picoband® (ABIN7602177). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Axin 2/AXIN2 recombinant protein (Position: D614-D843).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Callahan, N., Modesto, A., Meira, R., Seymen, F., Patir, A., Vieira, A. R. Axis inhibition protein 2 (AXIN2) polymorphisms and tooth agenesis. Arch. Oral Biol. 54: 45-49, 2009. 2. Dong, X., Seelan, R. S., Qian, C., Mai, M., Liu, W. Genomic structure, chromosome mapping and expression analysis of the human AXIN2 gene. Cytogenet. Cell Genet. 93: 26-28, 2001. 3. Lammi, L., Arte, S., Somer, M., Jarvinen, H., Lahermo, P., Thesleff, I., Pirinen, S., Nieminen, P. Mutations in AXIN2 cause familial tooth agenesis and predispose to colorectal cancer. Am. J. Hum. Genet. 74: 1043-1050, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for AXIN2

Target: AXIN2 (Axin 2 (AXIN2))

Alternative Name: AXIN2

Background:

Synonyms: Ubiquitin-like-conjugating enzyme ATG3,6.3.2.-,Autophagy-related protein 3,APG3-like,hApg3,Protein PC3-96,ATG3,APG3, APG3L,

Tissue Specificity: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. .

Background: Axin-2 also known as axin-like protein (Axil) or axis inhibition protein 2 (AXIN2) or conductin is a protein that in humans is encoded by the AXIN2 gene. The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

Molecular Weight: 100 kDa

Gene ID: 8313

UniProt: Q9Y2T1

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling