PHKG1 antibody (AA 62-353)

Catalog No. ABIN7602190

The Rabbit Polyclonal anti-PHKG1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect PHKG1 in samples from Human.

Quick Overview for PHKG1 antibody (AA 62-353) (ABIN7602190)

Target: PHKG1 (phosphorylase Kinase, gamma 1 (Muscle) (PHKG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHKG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PHKG1 Antibody

Binding Specificity: AA 62-353

Purpose: Anti-PHKG1 Antibody Picoband®

Characteristics: Anti-PHKG1 Antibody Picoband® (ABIN7602190). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PHKG1 recombinant protein (Position: E62-R353). Human PHKG1 shares 93.8% and 94.2% amino acid (aa) sequence identity with mouse and rat PHKG1, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Burwinkel, B., Hu, B., Schroers, A., Clemens, P. R., Moses, S. W., Shin, Y. S., Pongratz, D., Vorgerd, M., Kilimann, M. W. Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases. Europ. J. Hum. Genet. 11: 516-526, 2003. 2. Chamberlain, J. S., vanTuinen, P., Reeves, A. A., Philip, B. A., Caskey, C. T. Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice. Proc. Nat. Acad. Sci. 84: 2886-2890, 1987. 3. Jones, T. A., da Cruz e Silva, E. F., Spurr, N. K., Sheer, D., Cohen, P. T. Localization of the gene encoding the catalytic gamma subunit of phosphorylase kinase to human chromosome bands 7p12-q21. Biochim. Biophys. Acta 1048: 24-29, 1990.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PHKG1

Target: PHKG1 (phosphorylase Kinase, gamma 1 (Muscle) (PHKG1))

Alternative Name: PHKG1

Background: Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform is an enzyme that in humans is encoded by the PHKG1 gene. This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 40 kDa

Gene ID: 5260

UniProt: Q16816

Pathways: Cellular Glucan Metabolic Process