TRIM16 antibody (AA 65-210)

Catalog No. ABIN7602226

The Rabbit Polyclonal anti-TRIM16 antibody has been validated for ELISA, WB, IHC, ICC and IF. It is suitable to detect TRIM16 in samples from Mouse and Rat.

Quick Overview for TRIM16 antibody (AA 65-210) (ABIN7602226)

Target: TRIM16 (Tripartite Motif Containing 16 (TRIM16))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM16 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-TRIM16 Antibody

Binding Specificity: AA 65-210

Purpose: Anti-TRIM16 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRIM16 Antibody Picoband® (ABIN7602226). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse TRIM16 recombinant protein (Position: D65-K210).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Beer, H.-D., Munding, C., Dubois, N., Mamie, C., Hohl, D., Werner, S. The estrogen-responsive B box protein: a novel regulator of keratinocyte differentiation. J. Biol. Chem. 277: 20740-20749, 2002. 2. Liu, H.-L. C., Golder-Novoselsky, E., Seto, M. H., Webster, L., McClary, J., Zajchowski, D. A. The novel estrogen-responsive B box protein (EBBP) gene is tamoxifen regulated in cells expressing an estrogen receptor DNA-binding domain mutant. Molec. Endocr. 12: 1733-1748, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TRIM16

Target: TRIM16 (Tripartite Motif Containing 16 (TRIM16))

Alternative Name: Trim16

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP,

Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Background: Tripartite motif-containing protein 16 is a protein that in humans is encoded by the TRIM16 gene. This gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. The protein encoded by this gene contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth, differentiation and pathogenesis. Expression of this gene was detected in most tissues. Its function, however, has not yet been determined.

Molecular Weight: 63 kDa

Gene ID: 94092

Pathways: Retinoic Acid Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Growth Factor Binding