SH3G2 antibody (AA 65-292)

Catalog No. ABIN7602228

This Mouse Monoclonal antibody specifically detects SH3G2 in WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for SH3G2 antibody (AA 65-292) (ABIN7602228)

Target: SH3G2 (Endophilin-A1 (SH3G2))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SH3G2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 6I8E1

Product Details anti-SH3G2 Antibody

Binding Specificity: AA 65-292

Purpose: Anti-SH3GL2 Antibody Picoband® (monoclonal, 6I8E1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SH3GL2 Antibody Picoband® (monoclonal, 6I8E1) (ABIN7602228). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SH3GL2 recombinant protein (Position: R65-Q292).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
1. Gallop, J. L., Butler, P. J. G., McMahon, H. T. Endophilin and CtBP/BARS are not acyl transferases in endocytosis or Golgi fission. Nature 438: 675-678, 2005. 2. Giachino, C., Lantelme, E., Lanzetti, L., Saccone, S., Della Valle, G., Migone, N. A novel SH3-containing human gene family preferentially expressed in the central nervous system. Genomics 41: 427-434, 1997. 3. Howard, L., Nelson, K. K., Maciewicz, R. A., Blobel, C. P. Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1. J. Biol. Chem. 274: 31693-31699, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SH3G2

Target: SH3G2 (Endophilin-A1 (SH3G2))

Alternative Name: SH3GL2

Background:

Synonyms: 3-oxo-5-beta-steroid 4-dehydrogenase, Aldo-keto reductase family 1 member D1, Delta (4)-3-ketosteroid 5-beta-reductase, Delta (4)-3-oxosteroid 5-beta-reductase, AKR1D1, SRD5B1

Tissue Specificity: Highly expressed in liver. Expressed in testis and weakly in colon.

Background: Endophilin-A1 is a protein that in humans is encoded by the SH3GL2 gene. Endophilin proteins are part of a large family of Bin/Amphiphysin/Rvs (BAR) domain proteins that are involved in cell membrane remodeling. The endophilins are encoded by five genes, which produce endophilin A 1-3 and B 1-2. Endophilins are involved in many cellular mechanisms, such as synaptic vesicle recycling, receptor trafficking, and membrane remodeling processes. Research studies indicate that endophilin 1 (endophilin A1, SH3GL2) can induce different membrane shapes and participate in the morphogenesis of dendritic spines. Endophilin 1 is also involved in regulating blood brain barrier permeability via the EGFR-JNK pathway.

Molecular Weight: 40 kDa

Gene ID: 6456

UniProt: Q99962

Pathways: Neurotrophin Signaling Pathway, EGFR Downregulation