PHACTR1 antibody (AA 66-395)

Catalog No. ABIN7602246

Product Details anti-PHACTR1 Antibody

Target: PHACTR1 (Phosphatase and Actin Regulator 1 (PHACTR1))

Binding Specificity: AA 66-395

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHACTR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-PHACTR1 Antibody Picoband®

Characteristics: Anti-PHACTR1 Antibody Picoband® (ABIN7602246). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PHACTR1 recombinant protein (Position: K66-E395). Human PHACTR1 shares 94.8% and 95.2% amino acid (aa) sequence identity with mouse and rat PHACTR1, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Allen, P. B., Greenfield, A. T., Svenningsson, P., Haspeslagh, D. C., Greengard, P. Phactrs 1-4: a family of protein phosphatase 1 and actin regulatory proteins. Proc. Nat. Acad. Sci. 101: 7187-7192, 2004. 2. de Ligt, J., Willemsen, M. H., van Bon, B. W. M., Kleefstra, T., Yntema, H. G., Kroes, T., Vulto-van Silfhout, A. T., Koolen, D. A., de Vries, P., Gilissen, C., del Rosario, M., Hoischen, A., Scheffer, H., de Vries, B. B. A., Brunner, H. G., Veltman, J. A., Vissers, L. E. L. M. Diagnostic exome sequencing in persons with severe intellectual disability. New Eng. J. Med. 367: 1921-1929, 2012. 3. Hamada, N., Ogaya, S., Nakashima, M., Nishijo, T., Sugawara, Y., Iwamoto, I., Ito, H., Maki, Y., Shirai, K., Baba, S., Maruyama, K., Saitsu, H., Kato, M., Matsumoto, N., Momiyama, T., Nagata, K. De novo PHACTR1 mutations in West syndrome and their pathophysiological effects. Brain 141: 3098-3114, 2018.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PHACTR1

Target: PHACTR1 (Phosphatase and Actin Regulator 1 (PHACTR1))

Alternative Name: PHACTR1 (PHACTR1 Products)

Background: Phosphatase and actin regulator 1 (PHACTR1) is a protein that in humans is encoded by the PHACTR1 gene on chromosome 6. The protein encoded by this gene is a member of the phosphatase and actin regulator family of proteins. This family member can bind actin and regulate the reorganization of the actin cytoskeleton. It plays a role in tubule formation and in endothelial cell survival. Polymorphisms in this gene are associated with susceptibility to myocardial infarction, coronary artery disease and cervical artery dissection. Alternative splicing of this gene results in multiple transcript variants.

Molecular Weight: 69 kDa

Gene ID: 221692

UniProt: Q9C0D0