PTCD3 antibody (AA 66-648)

Catalog No. ABIN7602253

The Rabbit Polyclonal anti-PTCD3 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect PTCD3 in samples from Human, Mouse and Rat.

Quick Overview for PTCD3 antibody (AA 66-648) (ABIN7602253)

Target: PTCD3 (Pentatricopeptide Repeat Domain 3 (PTCD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTCD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-PTCD3 Antibody

Binding Specificity: AA 66-648

Purpose: Anti-PTCD3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PTCD3 Antibody Picoband® (ABIN7602253). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PTCD3 recombinant protein (Position: D66-R648). Human PTCD3 shares 76.4% amino acid (aa) sequence identity with mouse PTCD3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Borna, N. N., Kishita, Y., Kohda, M., Lim, S. C., Shimura, M., Wu, Y., Mogushi, K., Yatsuka, Y., Harashima, H., Hisatomi, Y., Fushimi, T., Ichimoto, K., Murayama, K., Ohtake, A., Okazaki, Y. Mitochondrial ribosomal protein PTCD3 mutations cause oxidative phosphorylation defects with Leigh syndrome. Neurogenetics 20: 9-25, 2019. 2. Davies, S. M. K., Rackham, O., Shearwood, A.-M. J., Hamilton, K. L., Narsai, R., Whelan, J., Filipovska, A. Pentatricopeptide repeat domain protein 3 associates with the mitochondrial small ribosomal subunit and regulates translation. FEBS Lett. 583: 1853-1858, 2009. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 11/6/2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PTCD3

Target: PTCD3 (Pentatricopeptide Repeat Domain 3 (PTCD3))

Alternative Name: PTCD3

Background:

Synonyms: PTCD3, MRPS39, TRG15, Pentatricopeptide repeat domain-containing protein 3, mitochondrial, 28S ribosomal protein S39, mitochondrial, MRP-S39, Mitochondrial small ribosomal subunit protein mS39, Transformation-related gene 15 protein, TRG-15

Background: Enables rRNA binding activity and ribosomal small subunit binding activity. Involved in mitochondrial translation. Located in several cellular components, including cytosol, mitochondrial matrix, and nucleoplasm. Implicated in combined oxidative phosphorylation deficiency 51.

Molecular Weight: 79 kDa

Gene ID: 55037