PRELP antibody (AA 69-353)

Catalog No. ABIN7602298

The Rabbit Polyclonal anti-PRELP antibody has been validated for WB, ELISA and FACS. It is suitable to detect PRELP in samples from Human, Mouse and Rat.

Quick Overview for PRELP antibody (AA 69-353) (ABIN7602298)

Target: PRELP (Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRELP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PRELP Antibody

Binding Specificity: AA 69-353

Purpose: Anti-PRELP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRELP Antibody Picoband® (ABIN7602298). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRELP recombinant protein (Position: I69-R353).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bengtsson, E., Neame, P. J., Heinegard, D., Sommarin, Y. The primary structure of a basic leucine-rich repeat protein, PRELP, found in connective tissues. J. Biol. Chem. 270: 25639-25644, 1995. 2. Grover, J., Chen, X.-N., Korenberg, J. R., Recklies, A. D., Roughley, P. J. The gene organization, chromosome location, and expression of a 55- kDa matrix protein (PRELP) of human articular cartilage. Genomics 38: 109-117, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRELP

Target: PRELP (Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP))

Alternative Name: PRELP

Background:

Synonyms: Cadherin-4, Retinal cadherin, R-CAD, R-cadherin, CDH4

Tissue Specificity: Expressed mainly in brain but also found in other tissues.

Background: Prolargin is a protein that in humans is encoded by the PRELP gene. The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed.

Molecular Weight: 65 kDa

Gene ID: 5549

UniProt: P51888

Pathways: Glycosaminoglycan Metabolic Process