BRD9 antibody (AA 7-271)

Catalog No. ABIN7602322

The Rabbit Polyclonal anti-BRD9 antibody has been validated for WB, ELISA and FACS. It is suitable to detect BRD9 in samples from Human, Mouse and Rat.

Quick Overview for BRD9 antibody (AA 7-271) (ABIN7602322)

Target: BRD9 (Bromodomain Containing 9 (BRD9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-BRD9 Antibody

Binding Specificity: AA 7-271

Purpose: Anti-BRD9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-BRD9 Antibody Picoband® (ABIN7602322). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BRD9 recombinant protein (Position: K7-K271).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Filippakopoulos, P., Picaud, S., Mangos, M., Keates, T., Lambert, J.-P., Barsyte-Lovejoy, D., Felletar, I., Volkmer, R., Muller, S., Pawson, T., Gingras, A.-C., Arrowsmith, C. H., Knapp, S. Histone recognition and large-scale structural analysis of the human bromodomain family. Cell 149: 214-231, 2012. 2. Flynn, E. M., Huang, O. W., Poy, F., Oppikofer, M., Bellon, S. F., Tang, Y., Cochran, A. G. A subset of human bromodomains recognizes butyryllysine and crotonyllysine histone peptide modifications. Structure 23: 1801-1814, 2015. 3. Gross, M. B. Personal Communication. Baltimore, Md. 6/10/2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for BRD9

Target: BRD9 (Bromodomain Containing 9 (BRD9))

Alternative Name: BRD9

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 44, Deubiquitinating enzyme 44, Ubiquitin thioesterase 44, Ubiquitin-specific-processing protease 44, USP44

Tissue Specificity: Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia.

Background: Bromodomain-containing protein 9 is a protein that in humans is encoded by the BRD9 gene. Enables lysine-acetylated histone binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of SWI/SNF complex.

Molecular Weight: 80 kDa

Gene ID: 65980

UniProt: Q9H8M2