ELISA, Western Blotting (WB), Flow Cytometry (FACS)
Purpose
Anti-IRGQ Antibody Picoband®
Characteristics
Anti-IRGQ Antibody Picoband® (ABIN7602340). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Purification
Immunogen affinity purified.
Immunogen
E.coli-derived human IRGQ recombinant protein (Position: D7-Q623). Human IRGQ shares 78.2% amino acid (aa) sequence identity with mouse IRGQ.
IRGQ
Reactivity: Human
ELISA, WB, FACS, IHC
Host: Rabbit
Polyclonal
PE
Application Notes
Western blot, 0.25-0.5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL, - 1. Bekpen, C., Hunn, J. P., Rohde, C., Parvanova, I., Guethlein, L., Dunn, D. M., ... & Howard, J. C. (2005). The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage. Genome biology, 6, 1-18. 2. Maekawa, M., Hiyoshi, H., Nakayama, J., Kido, K., Sawasaki, T., Semba, K., ... & Higashiyama, S. (2019). Cullin-3/KCTD10 complex is essential for K27-polyubiquitination of EIF3D in human hepatocellular carcinoma HepG2 cells. Biochemical and Biophysical Research Communications, 516(4), 1116-1122. 3. Chino, H., Hatta, T., Natsume, T., & Mizushima, N. (2019). Intrinsically disordered protein TEX264 mediates ER-phagy. Molecular cell, 74(5), 909-921.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.