DKK1 antibody (AA 73-272)

Catalog No. ABIN7602383

This anti-DKK1 antibody is a Rabbit Polyclonal antibody detecting DKK1 in ELISA and IHC. Suitable for Rat and Mouse.

Quick Overview for DKK1 antibody (AA 73-272) (ABIN7602383)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKK1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-DKK1 Antibody

Binding Specificity: AA 73-272

Purpose: Anti-Dkk1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Dkk1 Antibody Picoband® (ABIN7602383). Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Dkk1 recombinant protein (Position: N73-H272).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Fedi, P., Bafico, A., Soria, A. N., Burgess, W. H., Miki, T., Bottaro, D. P., Kraus, M. H., Aaronson, S. A.Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.J. Biol. Chem. 274: 19465-19472, 1999. 2. Roessler, E., Du, Y., Glinka, A., Dutra, A., Niehrs, C., Muenke, M.The genomic structure, chromosome location, and analysis of the human DKK1 head inducer gene as a candidate for holoprosencephaly. Cytogenet. Cell Genet. 89: 220-224, 2000. 3. Tian, E., Zhan, F., Walker, R., Rasmussen, E., Ma, Y., Barlogie, B., Shaughnessy, J. D., Jr.The role of Wnt-signaling antagonist DKK1 in the development of osteolytic lesions in multiple myeloma.New Eng. J. Med. 349: 2483-2494, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: Dkk1

Background:

Synonyms: Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, DKK1, UNQ492/PRO1008

Tissue Specificity: Placenta.

Background: DKK1, Dickkopf-related protein 1, is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the DKK1 gene contains 4 exons. The DKK1 gene is mapped to 10q11.2 using FISH. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Molecular Weight: 38 kDa

Gene ID: 13380

UniProt: O54908

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation