RTF1 antibody (AA 77-705)

Catalog No. ABIN7602444

This Rabbit Polyclonal antibody specifically detects RTF1 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human and Monkey.

Quick Overview for RTF1 antibody (AA 77-705) (ABIN7602444)

Target: RTF1 (RNA polymerase-associated protein RTF1 homolog (RTF1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RTF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-RTF1 Antibody

Binding Specificity: AA 77-705

Purpose: Anti-RTF1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-RTF1 Antibody Picoband® (ABIN7602444). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RTF1 recombinant protein (Position: S77-K705).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Nagase, T., Seki, N., Ishikawa, K., Ohira, M., Kawarabayasi, Y., Ohara, O., Tanaka, A., Kotani, H., Miyajima, N., Nomura, N. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. DNA Res. 3: 321-329, 1996. 2. Warner, M. H., Roinick, K. L., Arndt, K. M. Rtf1 is a multifunctional component of the Paf1 complex that regulates gene expression by ing cotranscriptional histone modification. Molec. Cell. Biol. 27: 6103-6115, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RTF1

Target: RTF1 (RNA polymerase-associated protein RTF1 homolog (RTF1))

Alternative Name: RTF1

Background:

Synonyms: Protein phosphatase 1 regulatory subunit 14A,17 kDa PKC-potentiated inhibitory protein of PP1,Protein kinase C-potentiated inhibitor protein of 17 kDa,CPI-17,PPP1R14A,CPI17, PPP1INL,

Tissue Specificity: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .

Background: Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) is a protein that in humans is encoded by the RTF1 gene. This locus may represent a gene involved in regulation of transcription elongation and chromatin remodeling, based on studies of similar proteins in other organisms. The encoded protein may bind single-stranded DNA.

Molecular Weight: 100 kDa

Gene ID: 23168

UniProt: Q92541

Pathways: Stem Cell Maintenance