TRIM2 antibody (AA 78-398)

Catalog No. ABIN7602449

This anti-TRIM2 antibody is a Rabbit Polyclonal antibody detecting TRIM2 in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for TRIM2 antibody (AA 78-398) (ABIN7602449)

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TRIM2 Antibody

Binding Specificity: AA 78-398

Purpose: Anti-TRIM2 Antibody Picoband®

Characteristics: Anti-TRIM2 Antibody Picoband® (ABIN7602449). Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRIM2 recombinant protein (Position: Q78-E398). Human TRIM2 shares 99.4% and 98.8% amino acid (aa) sequence identity with mouse and rat TRIM2, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Balastik, M., Ferraguti, F., Pires-da Silva, A., Lee, T. H., Alvarez-Bolado, G., Lu, K. P., Gruss, P. Deficiency in ubiquitin ligase TRIM2 causes accumulation of neurofilament light chain and neurodegeneration. Proc. Nat. Acad. Sci. 105: 12016-12021, 2008. 2. Nagase, T., Ishikawa, K., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 31-39, 1998. 3. Pehlivan, D., Coban Akdemir, Z., Karaca, E., Bayram, Y., Jhangiani, S., Yildiz, E. P., Muzny, D., Uluc, K., Gibbs, R. A., Baylor-Hopkins Center for Mendelian Genomics, Elcioglu, N., Lupski, J. R., Harel, T. Exome sequencing reveals homozygous TRIM2 mutation in a patient with early onset CMT and bilateral vocal cord paralysis. Hum. Genet. 134: 671-673, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRIM2

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Alternative Name: TRIM2

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. It plays a neuroprotective role and functions as an E3-ubiquitin ligase in proteasome-mediated degradation of target proteins. Mutations in this gene can cause early-onset axonal neuropathy. Alternative splicing results in multiple transcript variants.

Molecular Weight: 82 kDa

Gene ID: 23321

UniProt: Q9C040