RASGRP1 antibody (AA 8-678)

Catalog No. ABIN7602498

Product Details anti-RASGRP1 Antibody

Target: RASGRP1 (RAS Guanyl Releasing Protein 1 (Calcium and DAG-Regulated) (RASGRP1))

Binding Specificity: AA 8-678

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-RASGRP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RASGRP1 Antibody Picoband® (ABIN7602498). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RASGRP1 recombinant protein (Position: R8-Q678).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bivona, T. G., Perez de Castro, I., Ahearn, I. M., Grana, T. M., Chiu, V. K., Lockyer, P. J., Cullen, P. J., Pellicer, A., Cox, A. D., Philips, M. R. Phospholipase C-gamma activates Ras on the Golgi apparatus by means of RasGRP1. Nature 424: 694-698, 2003. 2. Bottorff, D., Ebinu, J., Stone, J. C. RasGRP, a Ras activator: mouse and human cDNA sequences and chromosomal positions. Mammalian Genome 10: 358-361, 1999. 3. Ebinu, J. O., Bottorff, D. A., Chan, E. Y. W., Stang, S. L., Dunn, R. J., Stone, J. C. RasGRP, a Ras guanyl nucleotide-releasing protein with calcium- and diacylglycerol-binding motifs. Science 280: 1082-1086, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RASGRP1

Target: RASGRP1 (RAS Guanyl Releasing Protein 1 (Calcium and DAG-Regulated) (RASGRP1))

Alternative Name: RASGRP1 (RASGRP1 Products)

Background:

Synonyms: Endoglin, Cell surface MJ7/18 antigen, CD105, Eng, Edg,

Background: RAS guanyl-releasing protein 1 is a protein that in humans is encoded by the RASGRP1 gene. This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE).

Molecular Weight: 100 kDa

Gene ID: 10125

UniProt: O95267

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway