GLI1 antibody (AA 800-1057)

Catalog No. ABIN7602517

The Rabbit Polyclonal anti-GLI1 antibody has been validated for WB and ELISA. It is suitable to detect GLI1 in samples from Human, Mouse and Rat.

Quick Overview for GLI1 antibody (AA 800-1057) (ABIN7602517)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GLI1 Antibody

Binding Specificity: AA 800-1057

Purpose: Anti-GLI1 Antibody Picoband®

Characteristics: Anti-GLI1 Antibody Picoband® (ABIN7602517). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GLI1 recombinant protein (Position: Y800-Q1057). Human GLI1 shares 79.8% amino acid (aa) sequence identity with mouse GLI1.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Kinzler KW, Bigner SH, Bigner DD, Trent JM, Law ML, O'Brien SJ, Wong AJ, Vogelstein B. (April 1987). "Identification of an amplified, highly expressed gene in a human glioma". Science 236 (4797): 70-3. 2. Ruiz i Altaba A. (June 1999). "Gli proteins encode context-dependent positive and negative functions: implications for development and disease.". Development 126 (14): 3205-16. 3. Sasaki H, Hui C, Nakafuku M, Kondoh H. (April 1997). "A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro". Development 124 (7): 1313-22.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI1

Background: GLI1, also known as GLI, is a protein originally isolated in human glioblastoma. The protein is encoded by GLI1 gene. It is mapped to 12q13.3. This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Beside, various upstream patterning signals may be integrated by the Gli proteins to a coherent programme of neurogenesis.

Molecular Weight: 150 kDa

Gene ID: 2735

UniProt: P08151

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis