TOM1 antibody (AA 84-480)

Catalog No. ABIN7602556

Product Details anti-TOM1 Antibody

Target: TOM1 (Target of Myb 1 (TOM1))

Binding Specificity: AA 84-480

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Anti-TOM1 Antibody Picoband®

Characteristics: Anti-TOM1 Antibody Picoband® (ABIN7602556). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TOM1 recombinant protein (Position: R84-K480). Human TOM1 shares 87.4% amino acid (aa) sequence identity with mouse TOM1.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Burk, O., Worpenberg, S., Haenig, B., Klempnauer, K. H. tom-1, a novel v-Myb target gene expressed in AMV- and E26-transformed myelomonocytic cells. EMBO J. 16: 1371-1380, 1997. 2. Katoh, Y., Imakagura, H., Futatsumori, M., Nakayama, K. Recruitment of clathrin onto endosomes by the Tom1-Tollip complex. Biochem. Biophys. Res. Commun. 341: 143-149, 2006. 3. Keskitalo, S., Haapaniemi, E. M., Glumoff, V., Liu, X., Lehtinen, V., Fogarty, C., Rajala, H., Chiang, S. C., Mustjoki, S., Kovanen, P., Lohi, J., Bryceson, Y. T., Seppanen, M., Kere, J., Heiskanen, K., Varjosalo, M. Dominant TOM1 mutation associated with combined immunodeficiency and autoimmune disease. NPJ Genomic Med. 4: 14, 2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TOM1

Target: TOM1 (Target of Myb 1 (TOM1))

Alternative Name: TOM1 (TOM1 Products)

Background: Target of Myb protein 1 is a protein that in humans is encoded by the TOM1 gene. This gene was identified as a target of the v-myb oncogene. The encoded protein shares its N-terminal domain in common with proteins associated with vesicular trafficking at the endosome. It is recruited to the endosomes by its interaction with endofin. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 54 kDa

Gene ID: 10043

UniProt: O60784