BCAT1 antibody (AA 88-386)

Catalog No. ABIN7602594

This Rabbit Polyclonal antibody specifically detects BCAT1 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BCAT1 antibody (AA 88-386) (ABIN7602594)

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-BCAT1 Antibody

Binding Specificity: AA 88-386

Purpose: Anti-BCAT1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-BCAT1 Antibody Picoband® (ABIN7602594). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BCAT1 recombinant protein (Position: L88-S386).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Naylor, S. L., Shows, T. B.Branched-chain aminotransferase genes (BCT-1 and BCT-2) assigned to human chromosomes 12 and 19 using alpha-keto acid selection media. (Abstract)Cytogenet. Cell Genet. 25: 191-192, 1979. 2. Jones, C., Moore, E. E.Isolation of mutants lacking branched-chain amino acid transaminase.Somat. Cell Genet. 2: 235-243, 1976.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for BCAT1

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Alternative Name: BCAT1

Background:

Synonyms: Sentrin-specific protease 6, SUMO-1-specific protease 1, Sentrin/SUMO-specific protease SENP6, SENP6, KIAA0797, SSP1, SUSP1, FKSG6

Tissue Specificity: Expressed in many tissues, highest levels in skeletal muscle.

Background: BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.

Molecular Weight: 43 kDa

Gene ID: 586

UniProt: P54687