nectin-3 antibody (AA 92-296)

Catalog No. ABIN7602666

This Rabbit Polyclonal antibody specifically detects nectin-3 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for nectin-3 antibody (AA 92-296) (ABIN7602666)

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This nectin-3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-nectin-3 Antibody

Binding Specificity: AA 92-296

Purpose: Anti-Nectin 3/NECTIN3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Nectin 3/NECTIN3 Antibody Picoband® (ABIN7602666). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Nectin 3/NECTIN3 recombinant protein (Position: E92-N296).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Inagaki, M., Irie, K., Ishizaki, H., Tanaka-Okamoto, M., Miyoshi, J., Takai, Y. Role of cell adhesion molecule nectin-3 in spermatid development. Genes Cells 11: 1125-1132, 2006. 2. Mizoguchi, A., Nakanishi, H., Kimura, K., Matsubara, K., Ozaki-Kuroda, K., Katata, T., Honda, T., Kiyohara, Y., Heo, K., Higashi, M., Tsutsumi, T., Sonoda, S., Ide, C., Takai, Y. Nectin: an adhesion molecule involved in formation of synapses. J. Cell Biol. 156: 555-565, 2002. 3. Reymond, N., Borg, J.-P., Lecocq, E., Adelaide, J., Campadelli-Fiume, G., Dubreuil, P., Lopez, M. Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin. Gene 255: 347-355, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for nectin-3

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Alternative Name: NECTIN3

Background:

Synonyms: Nectin-3, CDw113, Nectin cell adhesion molecule 3, Poliovirus receptor-related protein 3, CD113, NECTIN3, PRR3, PVRL3

Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Background: Nectin-3, also known as poliovirus receptor-related 3, is a 95 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is mapped to 3q13.13. This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of ional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants.

Molecular Weight: 80 kDa

Gene ID: 25945

Pathways: Cell-Cell Junction Organization