BAG1 antibody (C-Term)

Catalog No. ABIN7602774

This Rabbit Polyclonal antibody specifically detects BAG1 in WB. It exhibits reactivity toward Human.

Quick Overview for BAG1 antibody (C-Term) (ABIN7602774)

Target: BAG1 (BCL2-Associated Athanogene (BAG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAG1 Antibody

Binding Specificity: C-Term

Purpose: Anti-BAG1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-BAG1 Antibody Picoband® (ABIN7602774). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human BAG1, which shares 91.4% and 88.6% amino acid (aa) sequence identity with mouse and rat BAG1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
1. Takayama S, Sato T, Krajewski S, Kochel K, Irie S, Millan JA, Reed JC (Mar 1995). "Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity". Cell 80 (2): 279-84. 2. Takayama, S., Kochel, K., Irie, S., Inazawa, J., Abe, T., Sato, T., Druck, T., Huebner, K., Reed, J. C.Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12.Genomics35: 494-498, 1996. 3. "Entrez Gene: BAG1 BCL2-associated athanogene".

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for BAG1

Target: BAG1 (BCL2-Associated Athanogene (BAG1))

Alternative Name: BAG1

Background:

Synonyms: BAG family molecular chaperone regulator 1,BAG-1,Bcl-2-associated athanogene 1,BAG1,HAP,

Tissue Specificity: Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level). .

Background: BAG family molecular chaperone regulator 1 (BAG1) is a protein that in humans is encoded by the BAG1 gene. Human BAG1 is mapped to chromosome 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death. The Bag1 protein is rich in glutamic acid residues. Its deduced 274-amino acid protein has a calculated molecular mass of 31 KD. Being the BCL-2-associated athanogene, Bag1 enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms.

Molecular Weight: 33 kDa(S), 46 kDa(M), 56 kDa(L)

Gene ID: 573

UniProt: Q99933