ELP3/KAT9 antibody (C-Term)

Catalog No. ABIN7602789

This Mouse Monoclonal antibody specifically detects ELP3/KAT9 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ELP3/KAT9 antibody (C-Term) (ABIN7602789)

Target: ELP3/KAT9 (ELP3) (Elongator complex protein 3 (ELP3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ELP3/KAT9 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 8B4

Product Details anti-ELP3/KAT9 Antibody

Binding Specificity: C-Term

Purpose: Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) (ABIN7602789). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human KAT9/ELP3, identical to the related mouse sequence.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
1. Hawkes NA, Otero G, Winkler GS, Marshall N, Dahmus ME, Krappmann D, Scheidereit C, Thomas CL, Schiavo G, Erdjument-Bromage H, Tempst P, Svejstrup JQ (Jan 2002). "Purification and characterization of the human elongator complex". J Biol Chem. 277 (4): 3047-52. 2. Creppe C, Malinouskaya L, Volvert ML, Gillard M, Close P, Malaise O, Laguesse S, Cornez I, Rahmouni S, Ormenese S, Belachew S, Malgrange B, Chapelle JP, Siebenlist U, Moonen G, Chariot A, Nguyen L (Feb 2009). "Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin". Cell. 136 (3): 551-64.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ELP3/KAT9

Target: ELP3/KAT9 (ELP3) (Elongator complex protein 3 (ELP3))

Alternative Name: ELP3

Background:

Synonyms: Cystatin-B, CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB, CST6, STFB

Tissue Specificity: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.

Background: Elongator complex protein 3, also named KAT9, is aproteinthat in humans is encoded by theELP3gene. It is mapped to 8p21.1. ELP3 is the catalytichistone acetyltransferasesubunit of theRNA polymeraseII elongator complex, which is a component of the RNA polymerase II (Pol II)holoenzymeand is involved in transcriptional elongation. ELP3 supports the migration and branching ofprojection neuronsthroughacetylationof alpha-tubulin in the developingcerebral cortex. In mammals, ELP3 is important for paternalDNA demethylationafterfertilization.ELP3 is potentially involved in cellular redoxhomeostasisby mediating the acetylation ofglucose-6-phosphate dehydrogenase. Besides, ELP3 may play a role inchromatin remodelingand is involved in acetylation ofhistones H3and probablyH4.

Molecular Weight: 62 kDa

Gene ID: 55140

UniProt: Q9H9T3