ACADSB antibody (C-Term)

Catalog No. ABIN7602837

This Rabbit Polyclonal antibody specifically detects ACADSB in WB, IF, ICC and FACS. It exhibits reactivity toward Human, Rat, Mouse and Monkey.

Quick Overview for ACADSB antibody (C-Term) (ABIN7602837)

Target: ACADSB (Acyl-CoA Dehydrogenase, Short/branched Chain (ACADSB))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACADSB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ACADSB Antibody

Binding Specificity: C-Term

Purpose: Anti-SBCAD/ACADSB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SBCAD/ACADSB Antibody Picoband® (ABIN7602837). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SBCAD/ACADSB, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
1. Alfardan, J., Mohsen, A.-W., Copeland, S., Ellison, J., Keppen-Davis, L., Rohrbach, M., Powell, B. R., Gillis, J., Matern, D., Kant, J., Vockley, J. Characterization of new ACADSB gene sequence mutations and clinical implications in patients with 2-methylbutyrylglycinuria identified by newborn screening. Molec. Genet. Metab. 100: 333-338, 2010. 2. Andresen, B. S., Christensen, E., Corydon, T. J., Bross, P., Pilgaard, B., Wanders, R. J. A., Ruiter, J. P. N., Simonsen, H., Winter, V., Knudsen, I., Schroeder, L. D., Gregersen, N., Skovby, F. Isolated 2-methylbutyrylglycinuria caused by short/branched-chain acyl-CoA dehydrogenase deficiency: identification of a new enzyme defect, resolution of its molecular basis, and evidence for distinct acyl-CoA dehydrogenases in isoleucine and valine metabolism. Am. J. Hum. Genet. 67: 1095-1103, 2000. 3. Arden, K. C., Viars, C. S., Fu, K., Rozen, R. Localization of short/branched chain acyl-CoA dehydrogenase (ACADSB) to human chromosome 10. Genomics 25: 743-745, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ACADSB

Target: ACADSB (Acyl-CoA Dehydrogenase, Short/branched Chain (ACADSB))

Alternative Name: ACADSB

Background:

Synonyms: Annexin A8 ,Annexin VIII ,Annexin-8 ,Vascular anticoagulant-beta ,VAC-beta ,ANXA8 ,ANX8,

Tissue Specificity: Ubiquitously expressed.

Background: ACADSB is a human gene that encodes short/branched chain specific acyl-CoA dehydrogenase (SBCAD), an enzyme in the acyl CoA dehydrogenase family.Short/branched chain acyl-CoA dehydrogenase (ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa.

Molecular Weight: 40-44 kDa

Gene ID: 36

UniProt: P45954

Pathways: Monocarboxylic Acid Catabolic Process