TNNI3 antibody (C-Term)

Catalog No. ABIN7602860

This anti-TNNI3 antibody is a Rabbit Polyclonal antibody detecting TNNI3 in WB. Suitable for Mouse and Rat.

Quick Overview for TNNI3 antibody (C-Term) (ABIN7602860)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TNNI3 Antibody

Binding Specificity: C-Term

Purpose: Anti-Cardiac Troponin I/TNNI3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cardiac Troponin I/TNNI3 Antibody Picoband® (ABIN7602860). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cardiac Troponin I/TNNI3, which shares 95.5% amino acid (aa) sequence identity with mouse and rat TNNI3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
1. Arad, M., Penas-Lado, M., Monserrat, L., Maron, B. J., Sherrid, M., Ho, C. Y., Barr, S., Karim, A., Olson, T. M., Kamisago, M., Seidman, J. G., Seidman, C. E. Gene mutations in apical hypertrophic cardiomyopathy. Circulation 112: 2805-2811, 2005. 2. Barton, P. J. R., Cullen, M. E., Townsend, P. J., Brand, N. J., Mullen, A. J., Norman, D. A. M., Bhavsar, P. K., Yacoub, M. H. Close physical linkage of human troponin genes: organization, sequence, and expression of the locus encoding cardiac troponin I and slow skeletal troponin T. Genomics 57: 102-109, 1999. 3. Bermingham, N., Hernandez, D., Balfour, A., Gilmour, F., Martin, J. E., Fisher, E. M. C. Mapping TNNC1, the gene that encodes cardiac troponin I in the human and mouse. Genomics 30: 620-622, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: TNNI3

Background:

Synonyms: Alkaline phosphatase, placental type,3.1.3.1,Alkaline phosphatase Regan isozyme,Placental alkaline phosphatase 1,PLAP-1,ALPP,PLAP,

Tissue Specificity: Detected in placenta (at protein level). .

Background: Troponin I, cardiac muscle is a protein that in humans is encoded by the TNNI3 gene. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19.

Molecular Weight: 24 kDa

Gene ID: 7137

UniProt: P19429