CCR5 antibody (C-Term)

Catalog No. ABIN7602862

The Rabbit Polyclonal anti-CCR5 antibody has been validated for WB. It is suitable to detect CCR5 in samples from Human.

Quick Overview for CCR5 antibody (C-Term) (ABIN7602862)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CCR5 Antibody

Binding Specificity: C-Term

Purpose: Anti-CCR5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CCR5 Antibody Picoband® (ABIN7602862). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at C-terminus of human CCR5, which shares 76.2% and 85.7% amino acid (aa) sequence identity with mouse and rat CCR5, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
1. Ahuja, S. K., Kulkarni, H., Catano, G., Agan, B. K., Camargo, J. F., He, W., O'Connell, R. J., Marconi, V. C., Delmar, J., Eron, J., Clark, R. A., Frost, S., Martin, J., Ahuja, S. S., Deeks, S. G., Little, S., Richman, D., Hecht, F. M., Dolan, M. J. CCL3L1-CCR5 genotype influences durability of immune recovery during antiretroviral therapy of HIV-1-infected individuals. Nature Med. 14: 413-420, 2008. 2. Algood, H. M. S., Flynn, J. L. CCR5-deficient mice control Mycobacterium tuberculosis infection despite increased pulmonary lymphocytic infiltration. J. Immun. 173: 3287-3296, 2004. 3. Aliberti, J., Valenzuela, J. G., Carruthers, V. B., Hieny, S., Andersen, J., Charest, H., Reis e Sousa, C., Fairlamb, A., Ribeiro, J. M., Sher, A. Molecular mimicry of a CCR5 binding-domain in the microbial activation of dendritic cells. Nature Immun. 4: 485-490, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CCR5

Background:

Synonyms: C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195, CCR5, CMKBR5

Tissue Specificity: Epressed in mature thymocytes. Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines.

Background: C-C chemokine receptor type 5, also known as CCR5 or CD195, is a protein on the surface of white blood cells that is involved in the immune system as it acts as a receptor for chemokines. It is mapped to 3p21.31. This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles, the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 41 kDa

Gene ID: 1234

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size