FOXL2 antibody (C-Term)

Catalog No. ABIN7602885

This Rabbit Polyclonal antibody specifically detects FOXL2 in WB, FACS and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FOXL2 antibody (C-Term) (ABIN7602885)

Target: FOXL2 (Forkhead Box L2 (FOXL2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXL2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-FOXL2 Antibody

Binding Specificity: C-Term

Purpose: Anti-FOXL2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FOXL2 Antibody Picoband® (ABIN7602885). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human FOXL2, identical to the related mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Aparicio, S., Chapman, J., Stupka, E., Putnam, N., Chia, J., Dehal, P., Christoffels, A., Rash, S., Hoon, S., Smit, A., Gelpke, M. D. S., Roach, J., and 29 others. Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes. Science 297: 1301-1310, 2002. 2. Benayoun, B. A., Batista, F., Auer, J., Dipietromaria, A., L'Hote, D., De Baere, E., Veitia, R. A. Positive and negative feedback regulates the transcription factor FOXL2 in response to cell stress: evidence for a regulatory imbalance induced by disease-causing mutations. Hum. Molec. Genet. 18: 632-644, 2009. 3. Benayoun, B. A., Caburet, S., Dipietromaria, A., Bailly-Bechet, M., Batista, F., Fellous, M., Vaiman, D., Veitia, R. A. The identification and characterization of a FOXL2 response element provides insights into the pathogenesis of mutant alleles. Hum. Molec. Genet. 17: 3118-3127, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for FOXL2

Target: FOXL2 (Forkhead Box L2 (FOXL2))

Alternative Name: FOXL2

Background:

Synonyms: DNA dC->dU-editing enzyme APOBEC-3A, A3A, Phorbolin-1, APOBEC3A

Tissue Specificity: Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia.

Background: The forkhead transcription factor gene, FOXL2 located in blepharophimosis-ptosis-epicanthus inversus syndrome(BPES) critical region on chromosome 3q23. Consistent with an involvement in BPES, FOXL2 is selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles, in adult humans, it appears predominantly in the ovary. FOXL2 haploinsufficiency may cause BPES types I and II by the effect of a null allele and a hypomorphic allele, respectively. Furthermore, in a fraction of the BPES patients the genetic defect does not reside within the coding region of the FOXL2 gene and may be caused by a position effect. FOXL2 mutations can also cause gonadal dysgenesis or premature ovarian failure(POF) in women, as well as eyelid/forehead dysmorphology in both sexes.

Molecular Weight: 50 kDa

Gene ID: 668

UniProt: P58012

Pathways: Nuclear Hormone Receptor Binding, Positive Regulation of Endopeptidase Activity