PACAP antibody (C-Term)

Catalog No. ABIN7602909

Product Details anti-PACAP Antibody

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PACAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Anti-PACAP-38/ADCYAP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PACAP-38/ADCYAP1 Antibody Picoband® (ABIN7602909). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PACAP-38/ADCYAP1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Dorus, S., Vallender, E. J., Evans, P. D., Anderson, J. R., Gilbert, S. L., Mahowald, M., Wyckoff, G. J., Malcom, C. M., Lahn, B. T. Accelerated evolution of nervous system genes in the origin of Homo sapiens. Cell 119: 1027-1040, 2004. 2. Freson, K., Hashimoto, H., Thys, C., Wittevrongel, C., Danloy, S., Morita, Y., Shintani, N., Tomiyama, Y., Vermylen, J., Hoylaerts, M. F., Baba, A., Van Geet, C. The pituitary adenylate cyclase-activating polypeptide is a physiological inhibitor of platelet activation. J. Clin. Invest. 113: 905-912, 2004. 3. Hamelink, C., Tjurmina, O., Damadzic, R., Young, W. S., Weihe, E., Lee, H.-W., Eiden, L. E. Pituitary adenylate cyclase-activating polypeptide is a sympathoadrenal neurotransmitter involved in catecholamine regulation and glucohomeostasis. Proc. Nat. Acad. Sci. 99: 461-466, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PACAP

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Alternative Name: ADCYAP1 (ADCYAP1 Products)

Background:

Synonyms: Fibroblast growth factor 10, FGF-10, Keratinocyte growth factor 2, FGF10

Tissue Specificity: Expressed in numerous tissues with highest level in testis.

Background: Pituitary adenylate cyclase-activating polypeptide also known as PACAP is a protein that in humans is encoded by the ADCYAP1 gene. This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants.

Molecular Weight: 19 kDa

Gene ID: 116

UniProt: P18509

Pathways: Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Activity, cAMP Metabolic Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Regulation of G-Protein Coupled Receptor Protein Signaling