SAP30 antibody (C-Term)

Catalog No. ABIN7602922

The Rabbit Polyclonal anti-SAP30 antibody has been validated for WB and FACS. It is suitable to detect SAP30 in samples from Human.

Quick Overview for SAP30 antibody (C-Term) (ABIN7602922)

Target: SAP30 (Sin3A-Associated Protein, 30kDa (SAP30))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAP30 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-SAP30 Antibody

Binding Specificity: C-Term

Purpose: Anti-SAP30 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SAP30 Antibody Picoband® (ABIN7602922). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SAP30.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Gross, M. B. Personal Communication. Baltimore, Md. 7/25/2012. 2. Laherty, C. D., Billin, A. N., Lavinsky, R. M., Yochum, G. S., Bush, A. C., Sun, J.-M., Mullen, T.-M., Davie, J. R., Rose, D. W., Glass, C. K., Rosenfeld, M. G., Ayer, D. E., Eisenman, R. N. SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors. Molec. Cell. 2: 33-42, 1998. 3. Zhang, Y., Sun, Z.-W., Iratni, R., Erdjument-Bromage, H., Tempst, P., Hampsey, M., Reinberg, D. SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex. Molec. Cell 1: 1021-1031, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SAP30

Target: SAP30 (Sin3A-Associated Protein, 30kDa (SAP30))

Alternative Name: SAP30

Background:

Synonyms: Phospholipid-transporting ATPase IG (EC:7.6.2.1), ATPase IQ, ATPase class VI type 11C, P4-ATPase flippase complex alpha subunit ATP11C, ATP11C, ATPIG, ATPIQ

Tissue Specificity: Widely expressed.

Background: Sin3A-associated protein, 30 kDa, also known as SAP30, is a protein which in humans is encoded by the SAP30 gene. Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3.

Molecular Weight: 28 kDa

Gene ID: 8819

UniProt: O75446