TJP2 antibody (C-Term)

Catalog No. ABIN7602934

The Rabbit Polyclonal anti-TJP2 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect TJP2 in samples from Human, Rat and Monkey.

Quick Overview for TJP2 antibody (C-Term) (ABIN7602934)

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Reactivity: Human, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-TJP2 Antibody

Binding Specificity: C-Term

Purpose: Anti-TJP2/ZO2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TJP2/ZO2 Antibody Picoband® (ABIN7602934). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TJP2/ZO2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Duclos, F., Rodius, F., Wrogemann, K., Mandel, J.-L., Koenig, M. The Friedreich ataxia region: characterization of two novel genes and reduction of the critical region to 300 kb. Hum. Molec. Genet. 3: 909-914, 1994. 2. Itoh, M., Furuse, M., Morita, K., Kubota, K., Saitou, M., Tsukita, S. binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. J. Cell Biol. 147: 1351-1363, 1999. 3. Walsh, T., Pierce, S. B., Lenz, D. R., Brownstein, Z., Dagan-Rosenfeld, O., Shahin, H., Roeb, W., McCarthy, S., Nord, A. S., Gordon, C. R., Ben-Neriah, Z., Sebat, J., Kanaan, M., Lee, M. K., Frydman, M., King, M.-C., Avraham, K. B. Genomic duplication and overexpression of TJP2/ZO-2 leads to altered expression of apoptosis genes in progressive nonsyndromic hearing loss DFNA51. Am. J. Hum. Genet. 87: 101-109, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TJP2

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Alternative Name: TJP2

Background:

Synonyms: CD3 antigen, epsilon polypeptide, CD3e molecule, Cd3e, Cd3e_predicted, rCG_58471

Background: TJP2(Tight Junction Protein 2), also known as Zona Occludens 2 or ZO2 is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins(TJPs) belong to a family of membrane-associated guanylate kinase(MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. Duclos et al.(1994) mapped the TJP2 gene telomeric to the Friedreich ataxia critical region on chromosome 9q13-q21. TJP2 lies about 70 kb centromeric to the X123 gene and is transcribed in the centromere-to-telomere ion. Using in vitro assays and immunoprecipitation studies, Itoh et al.(1999) showed that the mouse Tjp1, Tjp2, and Tjp3 PDZ1 domains interacted with the C-terminal cytoplasmic domains of Cldn1 through Cldn8. In the mouse inner ear, Walsh et al.(2010) found that Tjp2 expression decreased rapidly between E16.5 and age 1 week to a level in adult mice that was approximately 50 % of the level at birth(P0).

Molecular Weight: 150-170 kDa

Gene ID: 9414