14-3-3 eta antibody (C-Term)

Catalog No. ABIN7602945

This Rabbit Polyclonal antibody specifically detects 14-3-3 eta in WB, IF, IHC, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for 14-3-3 eta antibody (C-Term) (ABIN7602945)

Target: 14-3-3 eta (YWHAH)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 eta antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-14-3-3 eta Antibody

Binding Specificity: C-Term

Purpose: Anti-YWHAH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-YWHAH Antibody Picoband® (ABIN7602945). Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human YWHAH.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Ichimura-Ohshima, Y., Morii, K., Ichimura, T., Araki, K., Takahashi, Y., Isobe, T., Minoshima, S., Fukuyama, R., Shimizu, N., Kuwano, R. cDNA cloning and chromosome assignment of the gene for human brain 14-3-3 protein eta chain. J. Neurosci. Res. 31: 600-605, 1992. 2. Muratake, T., Hayashi, S., Ichikawa, T., Kumanishi, T., Ichimura, Y., Kuwano, R., Isobe, T., Wang, Y., Minoshima, S., Shimizu, N., Takahashi, Y. Structural organization and chromosomal assignment of the human 14-3-3-eta chain gene (YWHAH). Genomics 36: 63-69, 1996. 3. Park, E., Rawson, S., Li, K., Kim, B.-W., Ficarro, S. B., Gonzalez-Del Pino, G., Sharif, H., Marto, J. A., Jeon, H., Eck, M. J. Architecture of autoinhibited and active BRAF-MEK1-14-3-3 complexes. Nature 575: 545-550, 2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for 14-3-3 eta

Target: 14-3-3 eta (YWHAH)

Alternative Name: YWHAH

Background:

Synonyms: YWHAH, YWHA1, 14-3-3 protein eta, Protein AS1

Background: 14-3-3 protein eta also referred to as 14-3-3η is a protein that in humans is encoded by the YWHAH gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.

Molecular Weight: 28 kDa

Gene ID: 7533

UniProt: Q04917

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, C21-Steroid Hormone Metabolic Process, Myometrial Relaxation and Contraction