TBX4 antibody (Middle Region)

Catalog No. ABIN7602998

This anti-TBX4 antibody is a Rabbit Polyclonal antibody detecting TBX4 in WB, FACS, ICC and IF. Suitable for Human.

Quick Overview for TBX4 antibody (Middle Region) (ABIN7602998)

Target: TBX4 (T-Box 4 (TBX4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBX4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-TBX4 Antibody

Binding Specificity: Middle Region

Purpose: Anti-TBX4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TBX4 Antibody Picoband® (ABIN7602998). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human TBX4, which shares 81.8% amino acid (aa) sequence identity with mouse TBX4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Agulnik, S. I., Garvey, N., Hancock, S., Ruvinsky, I., Chapman, D. L., Agulnik, I., Bollag, R., Papaioannou, V., Silver, L. M. Evolution of mouse T-box genes by tandem duplication and cluster dispersion. Genetics 144: 249-254, 1996. 2. Bollag, R. J., Siegfried, Z., Cebra-Thomas, J. A., Garvey, N., Davison, E. M., Silver, L. M. An ancient family of embryonically expressed mouse genes sharing a conserved protein motif with the T locus. Nature Genet. 7: 383-389, 1994. 3. Bongers, E. M. F., Van Bokhoven, H., Van Thienen, M.-N., Kooyman, M. A. P., Van Beersum, S. E. C., Boetes, C., Knoers, N. V. A. M., Hamel, B. C. J. The small patella syndrome: description of five cases from three families and examination of possible allelism with familial patella aplasia-hypoplasia and nail-patella syndrome. (Letter) J. Med. Genet. 38: 209-213, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TBX4

Target: TBX4 (T-Box 4 (TBX4))

Alternative Name: TBX4

Background:

Synonyms: Inorganic pyrophosphatase 2, mitochondrial, Pyrophosphatase SID6-306, Pyrophosphate phospho-hydrolase 2, PPase 2, PPA2, HSPC124

Tissue Specificity: Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium.

Background: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human homolog of mouse Tbx4, which is closely linked to Tbx2 on mouse chromosome 11. Similarly this gene, like TBX2, maps to human chromosome 17. Expression studies in mouse and chicken show that Tbx4 is expressed in developing hindlimb, but not in forelimb buds, suggesting a role for this gene in regulating limb development and specification of limb identity.

Molecular Weight: 60 kDa

Gene ID: 9496

UniProt: P57082