YY1 antibody (Middle Region)

Catalog No. ABIN7603001

This anti-YY1 antibody is a Mouse Monoclonal antibody detecting YY1 in WB, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for YY1 antibody (Middle Region) (ABIN7603001)

Target: YY1 (YY1 Transcription Factor (YY1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This YY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 6H3E1

Product Details anti-YY1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-YY1 Antibody Picoband® (monoclonal, 6H3E1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-YY1 Antibody Picoband® (monoclonal, 6H3E1) (ABIN7603001). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human YY1, identical to the related mouse sequence.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Shi Y, Seto E, Chang LS, Shenk T (Oct 1991). "Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein". Cell 67 (2): 377-88. 2. Thiaville MM, Kim J (2011). "Oncogenic potential of yin yang 1 mediated through control of imprinted genes". Critical Reviews in Oncogenesis 16 (3-4): 199-209. 3. Zhu W, Lossie AC, Camper SA, Gumucio DL (Apr 1994). "Chromosomal localization of the transcription factor YY1 in the mouse and human". Mammalian Genome 5 (4): 234-6.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Alternative Name: YY1

Background:

Synonyms: Dihydrofolate reductase, DHFR

Tissue Specificity: Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.

Background: YY1 (Yin Yang 1) is a transcriptional repressor protein in humans that is encoded by the YY1 gene. YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Molecular Weight: 65 kDa

Gene ID: 7528

UniProt: P25490