MADCAM1 antibody (Middle Region)

Catalog No. ABIN7603009

The Rabbit Polyclonal anti-MADCAM1 antibody has been validated for IHC. It is suitable to detect MADCAM1 in samples from Human.

Quick Overview for MADCAM1 antibody (Middle Region) (ABIN7603009)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MADCAM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-MADCAM1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-MAdCAM1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MAdCAM1 Antibody Picoband® (ABIN7603009). Tested in IHC applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human MAdCAM1, which shares 56.5% amino acid (aa) sequence identity with mouse MAdCAM1.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
1. Briskin, M. J., McEvoy, L. M., Butcher, E. C. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. 2. Entrez Gene: mucosal vascular addressin cell adhesion molecule 1. 3. Leung, E., Berg, R. W., Langley, R., Greene, J., Raymond, L. A., Augustus, M., Ni, J., Carter, K. C., Spurr, N., Choo, K. H. A., Krissansen, G. W. Genomic organization, chromosomal mapping, and analysis of the 5-prime promoter region of the human MAdCAM-1 gene. Immunogenetics 46: 111-119, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Alternative Name: MADCAM1

Background:

Synonyms: Mucosal addressin cell adhesion molecule 1, MAdCAM-1, hMAdCAM-1, MADCAM1

Tissue Specificity: Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.

Background: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that in humans is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, this gene is mapped to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4 / beta7), L-selectin, and VLA-4 (alpha4 / beta1) on myeloid cells to leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.

Molecular Weight: 43 kDa

Gene ID: 8174

UniProt: Q13477