Claudin 2 antibody (Middle Region)

Catalog No. ABIN7603035

This Rabbit Polyclonal antibody specifically detects Claudin 2 in WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Claudin 2 antibody (Middle Region) (ABIN7603035)

Target: Claudin 2 (CLDN2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Claudin 2 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Claudin 2/CLDN2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Claudin 2/CLDN2 Antibody Picoband® (ABIN7603035). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Claudin 2/CLDN2, identical to the related mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat
1. Morita K, Furuse M, Fujimoto K, Tsukita S (Mar 1999). "Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands". Proc Natl Acad Sci U S A 96 (2): 511-6. 2. Sakaguchi, T., Gu, X., Golden, H. M., Suh, E., Rhoads, D. B., Reinecker, H.-C. Cloning of the human claudin-2 5-prime-flanking region revealed a TATA-less promoter with conserved binding sites in mouse and human for caudal-related homeodomain proteins and hepatocyte nuclear factor-1-alpha. J. Biol. Chem. 277: 21361-21370, 2002. 3. Muto, S., Hata, M., Taniguchi, J., Tsuruoka, S., Moriwaki, K., Saitou, M., Furuse, K., Sasaki, H., Fujimura, A., Imai, M., Kusano, E., Tsukita, S., Furuse, M. (2010). "Claudin-2-deficient mice are defective in the leaky and cation-selective paracellular permeability properties of renal proximal tubules". Proceedings of the National Academy of Sciences 107 (17): 8011-8016.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Claudin 2

Target: Claudin 2 (CLDN2)

Alternative Name: CLDN2

Background:

Synonyms: Collagen alpha-1 (XVII) chain, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2, 120 kDa linear IgA dermatosis antigen, Linear IgA disease antigen 1, LAD-1, 97 kDa linear IgA bullous dermatosis antigen, 97 kDa LAD antigen, 97-LAD, Linear IgA bullous disease antigen of 97 kDa, LABD97, COL17A1, BP180, BPAG2

Tissue Specificity: Detected in skin. In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells. Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.

Background: Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.

Molecular Weight: 25 kDa

Gene ID: 9075

UniProt: P57739

Pathways: Hepatitis C