ENO1 antibody (Middle Region)

Catalog No. ABIN7603044

This Rabbit Polyclonal antibody specifically detects ENO1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ENO1 antibody (Middle Region) (ABIN7603044)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ENO1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-ENO1 Antibody Picoband®

Characteristics: Anti-ENO1 Antibody Picoband® (ABIN7603044). Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ENO1.

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
1. Comings, D. E. Evidence for ancient tetraploidy and conservation of linkage groups in mammalian chromosomes. Nature 238: 455-457, 1972. 2. D'Ancona, G. G., Chern, C. J., Benn, P., Croce, C. M. Assignment of the human gene for enolase 1 to region pter-p36 of chromosome 1. Cytogenet. Cell Genet. 18: 327-332, 1977. 3. D'Ancona, G. G., Croce, C. M. Assignment of the gene for enolase to mouse chromosome 4 using somatic cell hybrids. Cytogenet. Cell Genet. 19: 1-6, 1977.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: ENO1

Background: This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Molecular Weight: 47 kDa

Gene ID: 2023

UniProt: P06733