Ghrelin antibody (Middle Region)

Catalog No. ABIN7603053

This Rabbit Polyclonal antibody specifically detects Ghrelin in IHC. It exhibits reactivity toward Human.

Quick Overview for Ghrelin antibody (Middle Region) (ABIN7603053)

Target: Ghrelin (GHRL)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ghrelin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-Ghrelin Antibody

Binding Specificity: Middle Region

Purpose: Anti-Ghrelin/GHRL Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Ghrelin/GHRL Antibody Picoband® (ABIN7603053). Tested in IHC applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Ghrelin/GHRL, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. Abizaid, A., Liu, Z.-W., Andrews, Z. B., Shanabrough, M., Borok, E., Elsworth, J. D., Roth, R. H., Sleeman, M. W., Picciotto, M. R., Tschop, M. H., Gao, X.-B., Horvath, T. L. Ghrelin modulates the activity and synaptic input organization of midbrain dopamine neurons while promoting appetite. J. Clin. Invest. 116: 3229-3239, 2006. 2. Andrews, Z. B., Liu, Z.-W., Wallingford, N., Erion, D. M., Borok, E., Friedman, J. M., Tschop, M. H., Shanabrough, M., Cline, G., Shulman, G. I., Coppola, A., Gao, X.-B., Horvath, T. L., Diano, S. UCP2 mediates ghrelin's action on NPY/AgRP neurons by lowering free radicals. Nature 454: 846-851, 2008. Note: Erratum: Nature 459: 736 only, 2009. 3. Chartrel, N., Alvear-Perez, R., Leprince, J., Iturrioz, X., Reaux-Le Goazigo, A., Audinot, V., Chomarat, P., Coge, F., Nosjean, O., Rodriguez, M., Galizzi, J. P., Boutin, J. A., Vaudry, H., Llorens-Cortes, C. Comment on 'Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake.' Science 315: 766 only, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Ghrelin

Target: Ghrelin (GHRL)

Alternative Name: GHRL

Background:

Synonyms: Fibroblast growth factor 10, FGF-10, Keratinocyte growth factor 2, FGF10

Tissue Specificity: Expressed in numerous tissues with highest level in testis.

Background: This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression.

Molecular Weight: 20 kDa

Gene ID: 51738

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour