GZMK antibody (Middle Region)

Catalog No. ABIN7603055

This anti-GZMK antibody is a Rabbit Polyclonal antibody detecting GZMK in IHC. Suitable for Human.

Quick Overview for GZMK antibody (Middle Region) (ABIN7603055)

Target: GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GZMK antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-GZMK Antibody

Binding Specificity: Middle Region

Purpose: Anti-Granzyme K/GZMK Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Granzyme K/GZMK Antibody Picoband® (ABIN7603055). Tested in IHC applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Granzyme K/GZMK, which shares 71.4% and 73.9% amino acid (aa) sequence identity with mouse and rat GZMK, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. Baker, E., Sayers, T. J., Sutherland, G. R., Smyth, M. J. The genes encoding NK cell granule serine proteases, human tryptase-2 (TRYP2) and human granzyme A (HFSP), both map to chromosome 5q11-q12 and define a new locus for cytotoxic lymphocyte granule tryptases. Immunogenetics 40: 235-237, 1994. 2. Przetak, M. M., Yoast, S., Schmidt, B. F. Cloning of cDNA for human granzyme 3. FEBS Lett. 364: 268-271, 1995. 3. Sayers, T. J., Lloyd, A. R., McVicar, D. W., O'Connor, M. D., Kelly, J. M., Carter, C. R. D., Wiltrout, T. A., Wiltrout, R. H., Smyth, M. J. Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3. J. Leuko. Biol. 59: 763-768, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GZMK

Target: GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))

Alternative Name: GZMK

Background:

Synonyms: ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, HuB, Nervous system-specific RNA-binding protein Hel-N1, ELAVL2, HUB

Tissue Specificity: Brain, neural-specific.

Background: Granzyme K is a protein that in humans is encoded by the GZMK gene. This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes

Molecular Weight: 41 kDa

Gene ID: 3003

UniProt: P49863