HSD17B3 antibody (Middle Region)

Catalog No. ABIN7603060

The Rabbit Polyclonal anti-HSD17B3 antibody has been validated for WB and FACS. It is suitable to detect HSD17B3 in samples from Human, Rat and Mouse.

Quick Overview for HSD17B3 antibody (Middle Region) (ABIN7603060)

Target: HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-HSD17B3 Antibody

Binding Specificity: Middle Region

Purpose: Anti-HSD17B3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HSD17B3 Antibody Picoband® (ABIN7603060). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HSD17B3, which shares 68.4% amino acid (aa) sequence identity with rat HSD17B3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Ademola Akesode, F., Meyer, W. J., III, Migeon, C. J. Male pseudohermaphroditism with gynaecomastia due to testicular 17-ketosteroid reductase deficiency. Clin. Endocr. 7: 443-452, 1977. 2. Andersson, S., Geissler, W. M., Wu, L., Davis, D. L., Grumbach, M. M., New, M. I., Schwarz, H. P., Blethen, S. L., Mendonca, B. B., Bloise, W., Witchel, S. F., Cutler, G. B., Jr., Griffin, J. E., Wilson, J. D., Russell, D. W. Molecular genetics and pathophysiology of 17-beta-hydroxysteroid dehydrogenase 3 deficiency. J. Clin. Endocr. Metab. 81: 130-136, 1996. 3. Bilbao, J. R., Loridan, L., Audi, L., Gonzalo, E., Castano, L. A novel missense (R80W) mutation in 17-beta-hydroxysteroid dehydrogenase type 3 gene associated with male pseudohermaphroditism. Europ. J. Endocr. 139: 330-333, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HSD17B3

Target: HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))

Alternative Name: HSD17B3

Background:

Synonyms: Sodium channel protein type 11 subunit alpha, Peripheral nerve sodium channel 5, PN5, Sensory neuron sodium channel 2, Sodium channel protein type XI subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.9, hNaN, SCN11A, SCN12A, SNS2

Tissue Specificity: Expressed in the dorsal root ganglia and trigeminal ganglia, olfactory bulb, hippocampus, cerebellar cortex, spinal cord, spleen, small intestine and placenta.

Background: 17β-Hydroxysteroid dehydrogenase 3 (17β-HSD3) is an enzyme that in humans is encoded by the HSD17B3 gene and is involved in androgen steroidogenesis. This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.

Molecular Weight: 40 kDa

Gene ID: 3293

UniProt: P37058

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis