PDCD4 antibody (Middle Region)

Catalog No. ABIN7603080

This Rabbit Polyclonal antibody specifically detects PDCD4 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for PDCD4 antibody (Middle Region) (ABIN7603080)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PDCD4 Antibody

Binding Specificity: Middle Region

Purpose: Anti-PDCD4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDCD4 Antibody Picoband® (ABIN7603080). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region human PDCD4, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Azzoni, L, Zatsepina, O, Abebe, B, Bennett, IM, Kanakaraj, P, Perussia, B (1998). "Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells". Journal of immunology 161 (7): 3493-500. 2. Kang MJ, Ahn HS, Lee JY, Matsuhashi S, Park WY (2002). "Up-regulation of PDCD4 in senescent human diploid fibroblasts". Biochemical and Biophysical Research Communications. 293 (1): 617-21.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4

Background:

Synonyms: Programmed cell death protein 4,Neoplastic transformation inhibitor protein,Nuclear antigen H731-like,Protein 197/15a,PDCD4,H731,

Tissue Specificity: Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. .

Background: Programmed cell death protein 4 (PDCD4) is a protein that in humans is encoded by the PDCD4 gene. It is mapped to 10q25.2. This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. It also encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.

Molecular Weight: 64 kDa

Gene ID: 27250