MT-ND2 antibody (N-Term)

Catalog No. ABIN7603106

Product Details anti-MT-ND2 Antibody

Target: MT-ND2 (Mitochondrially Encoded NADH Dehydrogenase 2 (MT-ND2))

Binding Specificity: N-Term

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MT-ND2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purpose: Anti-NADH2/Mtnd2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NADH2/Mtnd2 Antibody Picoband® (ABIN7603106). Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat NADH2/Mtnd2, which shares 80% amino acid (aa) sequence identity with mouse NADH2/Mtnd2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat
1. Anderson, S., Bankier, A. T., Barrell, B. G., de Bruijn, M. H. L., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F., Schreier, P. H., Smith, A. J. H., Staden, R., Young, I. G. Sequence and organization of the human mitochondrial genome. Nature 290: 457-465, 1981. 2. Arizmendi, J. M., Skehel, J. M., Runswick, M. J., Fearnley, I. M., Walker, J. E. Complementary DNA sequences of two 14.5 kDa subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Complementation of the primary structure of the complex FEBS Lett. 313: 80-84, 1992. 3. Attardi, G., Chomyn, A., Doolittle, R. F., Mariottini, P., Ragan, C. I. Seven unidentified reading frames of human mitochondrial DNA encode subunits of the respiratory chain NADH dehydrogenase. Cold Spring Harbor Symp. Quant. Biol. 1: 103-114, 1986.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MT-ND2

Target: MT-ND2 (Mitochondrially Encoded NADH Dehydrogenase 2 (MT-ND2))

Alternative Name: Mtnd2 (MT-ND2 Products)

Background:

Synonyms: NADH-ubiquinone oxidoreductase chain 2, NADH dehydrogenase subunit 2, Mtnd2, mt-Nd2, Nd2

Tissue Specificity: Ubiquitously expressed.

Background: Mitochondrially encoded NADH dehydrogenase 2 is protein that in humans is encoded by the mitochondrial gene MT-ND2 gene. The ND2 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variants of MT-ND2 are associated with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS), Leigh's syndrome (LS), Leber's hereditary optic neuropathy (LHON) and increases in adult BMI.

Molecular Weight: 39 kDa

Gene ID: 26194

UniProt: P11662