MAF antibody (N-Term)

Catalog No. ABIN7603123

The Rabbit Polyclonal anti-MAF antibody has been validated for WB, IHC and FACS. It is suitable to detect MAF in samples from Human, Mouse and Rat.

Quick Overview for MAF antibody (N-Term) (ABIN7603123)

Target: MAF (V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian) (MAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-MAF Antibody

Binding Specificity: N-Term

Purpose: Anti-c-Maf/MAF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-c-Maf/MAF Antibody Picoband® (ABIN7603123). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human c-Maf/MAF, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: "Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
"1. Nishizawa M, Kataoka K, Goto N, Fujiwara KT, Kawai S (Oct 1989). "v-maf, a viral oncogene that encodes a "leucine zipper" motif". Proceedings of the National Academy of Sciences of the United States of America. 86 (20): 7711-5. 2. Olbrot M, Rud J, Moss LG, Sharma A (May 2002). "Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA". Proceedings of the National Academy of Sciences of the United States of America. 99 (10): 6737-42. 3. Melloul D, Marshak S, Cerasi E (Mar 2002). "Regulation of insulin gene transcription". Diabetologia. 45 (3): 309-26.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAF

Target: MAF (V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian) (MAF))

Alternative Name: MAF

Background:

Synonyms: Transcription factor Maf, Proto-oncogene c-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog, MAF

Tissue Specificity: Expressed in endothelial cells.

Background: Transcription factor Maf also known as proto-oncogene c-Maf or V-maf musculoaponeurotic fibrosarcoma oncogene homolog is a transcription factor that in humans is encoded by the MAF gene. It is mapped to 16q23.2. The protein encoded by this gene is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in this gene are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4). Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 42-50 kDa

Gene ID: 4094

UniProt: O75444