MFN1 antibody (N-Term)

Catalog No. ABIN7603129

The Mouse Monoclonal anti-MFN1 antibody has been validated for WB, ICC and IF. It is suitable to detect MFN1 in samples from Human.

Quick Overview for MFN1 antibody (N-Term) (ABIN7603129)

Target: MFN1 (Mitofusin 1 (MFN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 3H3

Product Details anti-MFN1 Antibody

Binding Specificity: N-Term

Purpose: Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) (ABIN7603129). Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. Adams MD, Kerlavage AR, Fields C, Venter JC (Sep 1993). "3,400 new expressed sequence tags identify diversity of transcripts in human brain". Nat Genet 4 (3): 256-67. 2. Meeusen, S., McCaffery, J. M., Nunnari, J. Mitochondrial fusion intermediates revealed in vitro. Science 305: 1747-1752, 2004. 3. Santel, A., Fuller, M. T. Control of mitochondrial morphology by a human mitofusin. J. Cell Sci. 114: 867-874, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MFN1

Target: MFN1 (Mitofusin 1 (MFN1))

Alternative Name: MFN1

Background:

Synonyms: Fzo homolog antibody|Hfzo1 antibody|Hfzo2 antibody|MFN 1 antibody|Mfn1 antibody|MFN1_HUMAN antibody|Mitochondrial transmembrane GTPase Fzo 1 antibody|Mitochondrial transmembrane GTPase FZO 2 antibody|Mitochondrial transmembrane GTPaseFZO1B antibody|Mitofusin 1 antibody|Mitofusin-1 antibody|Mitofusin1 antibody|Putative transmembrane GTPase antibody|Transmembrane GTPase MFN1 antibody

Tissue Specificity: Ubiquitous.Expressed at slightly higher level in kidney and heart.Isoform 2 may be overexpressed in some tumors,such as lung cancers.

Background: Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80-90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN-2, and to undergo ubiquitination by MARCH5. MFN-1 has two key domains. One is a coiled-coil region that mediates MFN-1: MFN-1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.

Molecular Weight: 84 kDa

Gene ID: 55669