AFF4 antibody (N-Term)

Catalog No. ABIN7603136

This anti-AFF4 antibody is a Mouse Monoclonal antibody detecting AFF4 in WB and FACS. Suitable for Human.

Quick Overview for AFF4 antibody (N-Term) (ABIN7603136)

Target: AFF4 (AF4/FMR2 Family, Member 4 (AFF4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AFF4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 8G12

Product Details anti-AFF4 Antibody

Binding Specificity: N-Term

Purpose: Anti-AFF4 Picoband® Antibody (monoclonal, 8G12)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AFF4 Picoband® Antibody (monoclonal, 8G12) (ABIN7603136). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human AFF4, identical to the related mouse sequence.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Izumi, K., Nakato, R., Zhang, Z., Edmondson, A. C., Noon, S., Dulik, M. C., Rajagopalan, R., Venditti, C. P., Gripp, K., Samanich, J., Zackai, E. H., Deardorff, M. A., and 10 others. Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin. Nature Genet. 47: 338-344, 2015. 2. Taki, T., Kano, H., Taniwaki, M., Sako, M., Yanagisawa, M., Hayashi, Y. AF5q31, a newly identified AF4-related gene, is fused to MLL in infant acute lymphoblastic leukemia with ins(5,11)(q31,q31q23). Proc. Nat. Acad. Sci. 96: 14535-14540, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AFF4

Target: AFF4 (AF4/FMR2 Family, Member 4 (AFF4))

Alternative Name: AFF4

Background:

Synonyms: SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2, UBA2, SAE2, UBLE1B, HRIHFB2115

Background: The AFF4 gene encodes a scaffold protein that functions as a core component of the super elongation complex (SEC), which is involved in transcriptional regulation during embryogenesis. The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. This gene is mapped to chromosome 5q31.

Molecular Weight: 150 kDa

Gene ID: 27125