ARID3A antibody (N-Term)

Catalog No. ABIN7603197

This Rabbit Polyclonal antibody specifically detects ARID3A in WB, IP and FACS. It exhibits reactivity toward Human.

Quick Overview for ARID3A antibody (N-Term) (ABIN7603197)

Target: ARID3A (AT Rich Interactive Domain 3A (BRIGHT-Like) (ARID3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID3A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-ARID3A Antibody

Binding Specificity: N-Term

Purpose: Anti-ARID3A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ARID3A Antibody Picoband® (ABIN7603197). Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ARID3A, which shares 95.8% amino acid (aa) sequence identity with mouse ARID3A.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Alejo-Valle, O., Weigert, K., Bhayadia, R., Ng, M., Issa, H., Beyer, C., Emmrich, S., Schuschel, K., Ihling, C., Sinz, A., Zimmermann, M., Wickenhauser, C., Flasinski, M., Regenyi, E., Labuhn, M., Reinhardt, D., Yaspo, M. L., Heckl, D., Klusmann, J. H. The megakaryocytic transcription factor ARID3A suppresses leukemia pathogenesis. Blood 139: 651-665, 2022. 2. Herrscher, R. F., Kaplan, M. H., Lelsz, D. L., Das, C., Scheuermann, R., Tucker, P. W. The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family. Genes Dev. 9: 3067-3082, 1995. 3. Kortschak, R. D., Reimann, H., Zimmer, M., Eyre, H. J., Saint, R., Jenne, D. E. The human dead ringer/bright homolog, DRIL1: cDNA cloning, gene structure, and mapping to D19S886, a marker on 19p13.3 that is strictly linked to the Peutz-Jeghers syndrome. Genomics 51: 288-292, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ARID3A

Target: ARID3A (AT Rich Interactive Domain 3A (BRIGHT-Like) (ARID3A))

Alternative Name: ARID3A

Background:

Synonyms: ARID3A, DRIL1, DRIL3, DRX, E2FBP1, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A, B-cell regulator of IgH transcription, Bright, Dead ringer-like protein 1, E2F-binding protein 1

Background: AT-rich interactive domain-containing protein 3A is a protein that in humans is encoded by the ARID3A gene. This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.

Molecular Weight: 80 kDa

Gene ID: 1820

UniProt: Q99856

Pathways: Chromatin Binding