beta Actin antibody (N-Term) (HRP)

Catalog No. ABIN7603251

This Rabbit Polyclonal antibody specifically detects beta Actin in WB. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Monkey and Zebrafish (Danio rerio).

Quick Overview for beta Actin antibody (N-Term) (HRP) (ABIN7603251)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is conjugated to HRP

Application: Western Blotting (WB)

Product Details anti-beta Actin Antibody (HRP)

Binding Specificity: N-Term

Purpose: Anti-beta-Actin ACTB Antibody Picoband® (HRP)

Cross-Reactivity: Chicken, Human, Monkey, Mouse, Rat, Zebrafish (Danio rerio)

Cross-Reactivity (Details): HRP-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.

Characteristics: Anti-beta-Actin ACTB Antibody (HRP) (-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: HRP-Beta-Actin antibody is affinity chromatography purified via peptide column.

Immunogen: HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.02-0.1 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 0.5 mg/mL

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: HRP-Beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background:

Synonyms: HRP-Actin, Beta-Actin, Actin, Beta actin, b actin

Tissue Specificity: Widely expressed, with the highest expression in lung, spleen and thymus.

Background: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Sequence Similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

Molecular Weight: 42 kDa

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction