Recombinant Bassoon antibody (AA 157-517)

Catalog No. ABIN7607128

The Chicken Chimeric anti-Bassoon antibody has been validated for IHC, WB, ELISA and ICC. It is suitable to detect Bassoon in samples from Rat.

Quick Overview for Recombinant Bassoon antibody (AA 157-517) (ABIN7607128)

Target: Bassoon (BSN)

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Chicken, Mouse

Clonality: Chimeric

Conjugate: This Bassoon antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: L124-59

Product Details anti-Bassoon Antibody

Binding Specificity: AA 157-517

Purpose: Anti-Bassoon Recombinant Chicken Chimeric mAb (L124/59)

Specificity: No off-targets reported

Cross-Reactivity: Mouse, Rat

Characteristics: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L124/59 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L124/59 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Purification: Purified by affinity chromatography.

Immunogen: Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli

Isotype: IgY

Application Details

Application Notes: IHC: 1:500 ICC: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1X PBS, 0.05 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Target Details for Bassoon

Target: Bassoon (BSN)

Alternative Name: Bassoon

Molecular Weight: 400 kDa for full-length protein, many smaller bands possible depending on sample used

Gene ID: 29138