Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Purpose
Anti-Arl13b Recombinant Chicken Chimeric mAb (N295B/66)
Specificity
No off-targets reported for Arl13a (based on KO validation results)
Cross-Reactivity
Human, Mouse, Non-Human Primate, Rat, Zebrafish (Danio rerio)
Characteristics
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N295B/66 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N295B/66 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Purification
Purified by affinity chromatography.
Immunogen
Fusion protein amino acids 208-427 (C-terminus) of mouse Arl13b (accession number Q640N2) produced recombinantly in E. Coli
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.